Introduction: Disasters are situations that seriously threaten public health and require urgent intervention. In these processes, the role of healthcare personnel is critical, and effective interventions are directly life-saving. The self-efficacy demonstrated by healthcare personnel in disaster response is closely related to individual skills as well as professional experience and educational background. Therefore, determining the self-efficacy levels of healthcare workers who served in disasters is important for improving future disaster management processes. Purpose: The aim of this research is to determine the disaster response self-efficacy levels of healthcare personnel who have served in disasters. T he study was conducted with the purpose of enabling volunteer healthcare workers, who may take part in future disasters, to respond more effectively and to provide higher-quality services at both national and international levels. Findings: The research was carried out using a survey model; during the data collection process, the “Disaster Response Self-Efficacy Scale (DRSES)” and a “Socio-demographic Data Form” developed by the researcher were used. The findings revealed that professional boundaries defined the field duties of medical doctors, nurses, and health technicians/officers actively serving in disasters. In addition, it was determined that self-efficacy levels increased with age and professional experience. The study also found that the educational background of healthcare personnel was a significant determinant depending on their role during the disaster process. Conclusion: The results of the study showed that the disaster response self-efficacy levels of healthcare personnel were influenced by age, experience, and education. Accordingly, it is emphasized that regularly repeating pre-disaster training is a critical factor in strengthening the intervention skills of healthcare workers. Expanding regular and practical training programs will improve the quality of healthcare services provided during disasters.