This study aims to determine the level of satisfaction among patients receiving primary healthcare services and the job satisfaction levels of healthcare professionals providing these services, and to examine the relationship between them. The cross-sectional study was conducted in January 2020 in Doğanyol district of Malatya, Turkey, and involved 230 patients and 16 healthcare workers. The Turkish version of the EUROPEP scale was used to measure patient satisfaction, and the Minnesota Satisfaction Questionnaire was used to assess job satisfaction. No significant relationship was found between patients’ satisfaction levels and their gender or marital status, whereas a statistically significant association was observed between education level and satisfaction (p<0.05). A significant relationship was also found between satisfaction with the delivery of healthcare services and overall patient satisfaction (p<0.05). Among healthcare workers, no significant differences were found between job satisfaction and socio-demographic variables (p>0.05). Furthermore, job satisfaction and patient satisfaction were not significantly correlated (p>0.05). The findings suggest that patient satisfaction is a multidimensional phenomenon and cannot be explained solely by the job satisfaction of healthcare workers. Therefore, it is recommended that both patient satisfaction and job satisfaction of healthcare workers be regularly monitored in order to improve the quality and effectiveness of primary healthcare services.